The Effects Of Kudiezi Injection(KDZI) On Hemodynamics In Acute Myocardial Infarct Dogs

With the increasing of cultural life and material life, the sick rate of cerebrovascular disease and angiocardiopathy is rising. Also, the death rate is going up. Its development has a trend to harm the young people. It is very easy that cerebrovascular disease and angiocardiopathy can happened in our country. These diseases kill 200 million peoples every year in China. It has become the first killer.The reason of acute myocardial infarction is blood supplying of coronary artery decreased or stopped, which will make the myocardium lack of blood. The studying of acute myocardial infarction reduces the death rate. But, the treat of drug for the recover of the people who infect the disease of acute myocardial infarction is an important method. So it is very important work for all of the scientists to develop a safe and available antimyocardial ischemic drug.Kudiezi injection (KDZI) was separated from Ixeris Sonchifolia (Bge) Hance and is a preparation of pure traditional Chinese drug. The main components of KDZI are substance of adenosine and flavonoid. The acute myocardial infarct (AMI) model of ligated left anterior descending coronary artery (LAD) of a dog was made. The function and mechanism of protective effect of KDZI for myocardial function and myocardial blood flow (MBF) were studied. This research will provide a scientific basis for using the KDZI.After weighed, the dog was anaesthesia by injecting (30 mg/kg). Back of the dog was fixed on the operation table. After shearing the hair, the neck and thoracic cavity of the dog were cut off then a tube, which was connected to breath machine, was put into the dog,s trachea. The left vein was separated.Then a tube was put into coronary artery. The artery was separated then a tube was put in and connected to energy change machine. Using an amplifier (AP-601G), the systolic blood pressure (SBP) and diastolic blood pressure (DBP) was measured. The right vein was separated and put into a tube that connected to pump for supplying the drug. The left artery was separated and a tube was put into left heart ventricle. Then connected to amplifier (AP-601G) for measuring left ventricular pressure (LVP) and maximum change rate of LVP (+/-dp/dtmax). Connecting to power-driven breath machine (12-16 times/min), let the anaesthesied dog right prone position, then cut the thoracic cavity, take out the fourth rib on the left. Making the heart expose out using cut- thoracic machine, cut off the keeping film, made a keep heart suspended bed. Separating the root part of the arota, putting the electromagenetic flow detector, the cardiac output (CO) was measured. Separating the levoisomer of coronary artery under the left auricle, putting the suitable electromagenetic flow detector for measuring coronary blood flow (CBF). Separating the desceding branch at 1/3 point of main junction, then put a line for ligition. Using electrocardiograph connected to AB-601 and AT-601 amplifier to detect heart rate (HR). Using multipoint wet adsorption to assignment test the epicardium electrocardiogram (EECG). Assignment test point is 20, which were divided into normal part, edge part of choking and centre part of choking. After operation, the dog was kept 15 minute steady. At the end of breath every time, record the all of the date that is taken before eating drug by RM-6000 multichannel physiological recording system. The EECG at all of the point were recorded using RM-6000 multichannel physiological recording system. The degree of ischemia (∑-ST)was expressed in total voltage before(0minute)ligition. The range of ischemia (N-ST) was expressed in lead number (≥2 mV)of ST section before ligition. After coronary artery was ligated 5 minutes, the drug was put into the sodium chloride solution (200 mL). The injection was carried out in constant speed and spent 90 minutes at right femoral vein. After injection, we measure the EECG at 30, 60, 90, 120, 180, 240, 300 and 360 minutes. Before measuring, the surface of the heart was cleaned. Demarcating the standard voltage, make the record paper 10 mm is equal to 5 mV. Calculating the total of∑-ST and N-ST when lead number (≥2 mV)of ST section. Calculating the difference of∑-ST (△∑-ST) and N-ST(△N-ST) between eating drug and after eating the drug. Repeat the same recording after LAD was broken off 15, 30, 60, 90, 120, 180, 240, 300, 360 minutes. We can calculate the average arterial pressure (MAP), myocardial blood flow (MBF), cardiac index (CI), coronary vascular resistance (CAR) and total peripheral resistance (TPR). Before and after the 120, 180, 240, 300, and 360 minutes of eating drug, the blood was collected from the coronary vein sinus and left femoral artery at the same time. The oxygen content was measured using blood gas analyzer of CORNIHG 178 type. According to the formula, which was used at the reference, we can calculate the oxygen consumption of myocardium (COC), oxygen utilize rate of myocardium (MOUR) and oxygen consumption index of myocardium (MOCI).The experiments show that KDZI can ease the degree of myocardial ischemia for a dog who was broken 6 hour with LAD, reduce the range of myocardial ischemia. It is very clear that KDZI can antagonize the reduce of blood pressure (SBP, DBP, MAP) when acute myocardial infarction happened. Also it can increase LVP, +/- p/dtmax, CO and CI. KDZI can improve thefunction of myocardium contraction and antagonize the pump failure. For coronary circulation, KDZI can increase clearly MBF, reduce the CVR. It can improve the myocardial blood supply. Moreover, KDZI can reduce clearly the utilization ratio of myocardial oxygen and oxygen consumption. Meanwhile, myocardial oxygen consumption index does not increase. Due to KDZI can clearly decrease AMI and LVEDP, it can reduce the heart load and the work. Also it can decrease the oxygen consumption in heart.