Study On Toxicological Characteristics And Hypoglycemic Action Of Tea Ployphenols Preparation

It is very important to prevent and cure the diabetic mellitus because of the high morbidity of the disease. To develop a new kind of functional tea-food with the hypoglycemic effect, the research investigated the toxicological characteristics and the suppressive hyperglycemic action of tea ployphenols preparation (TPP). The results of the study were as following:TPP was made by tea polyphenols, the extract of the fruit of privet, extract of mulberry leaves, extract of the fiveleaf gynostemma and other subsidiary materials that were for tablet making. The samples were made in line with the method of coated tablet processing. And the main functional components of it were tea polyphenols (TP≥19.07%) and polysaccharides (Ps≥10.07%).TPP was safe to be taken and there was no significant evident toxicity for orally intake. The result of acute toxicity test showed that, after being given TPP, no animals were dead and no any other harmful symptoms were observed. And the maximum tolerable dose (MTD) was larger than 20.0g/kg.BW. At the same time, the results of the 30-days-feeding test were negative, too. They showed that there was no significant influence on the weight gain, food utility ratio, appetite, hemogram index, blood biochemical index and organic weight/body weight of the rats. And there were no obviously harmful changes on the main visceral organs of the rats by observation of vivisection and tissue dissection.There's no significant genetic toxicity of TPP. All the results of Ames test, bone marrow cell micronucleus test in mice and sperm abnormality test in mice were negative.TPP had hypoglycemic action. After giving the normal and the diabetic mice induced by alloxan with TPP for 30 days, the results were got. And the results showed that, after 30 days' experiment, the body weight of the mice was not changed significantly (P>0.05). And compared with the control group, only the level of the group given TPP at the high dose (900mg/kg.BW) fell significantly by 22.00% (P<0.01), while the other two groups just showed the same tendency (P>0.05). At the same time, the glucose tolerance of the diabetic mice in the groups with three doses was improved significantly by using TPP (P<0.05), too. And all the normal mice given TPP were as healthy as before, and their body weight and level of blood glucose were not changed significantly (P>0.05).In a summary, all the results of the study showed that, TPP was safe to be taken orally and it had hypoglycemic action.