Study On The Metabolism And The Function In Vitro During The Storage Of Platelet At 22℃

At present, clinical platelet concentrates are stored at 22 ℃ for 5 days , and include plateletapheresis and buffy-coat-derived platelet concentrates . The quality standards on platelet are mainly limited in the platelet yields, residual leukocyte , residual erythrocyte and pH value, and lack the indices of the metabolism and the function in vitro during the storage, which causes the quality of platelet to be not uniform and impacts the effect of transfusion.The objective of this study was to understand the orderliness of metabolism and function in vitro during storage of platelet at 22℃ and analyze the correlation between them by detecting the metabolic and functional parameters of platelet during storage , and choose effective indices reflecting platelet function, and optimize the preparation technology of platelet, and provide bases for establishing whole quality evaluation system on platelet.1 , Study on the metabolism and the function in vitro during the storage ofplateletapheresisDuring the storage of plateletapheresis, lactate and H～+ were accumulated in plasm, and pH value gradually decreased with the consumption of oxygen; HSR remarkably decreased from day 7; CD62p expression incresed, and CD62p anew expression decreased with the prolong of storage time; There were good correlations between the metabolic parameters(blood gases , glucose concentration , lactate concentration) and the functional characteristics(HSR, CD62p expression, CD62p anew expression) in vitro during the storage of plateletapheresis.We can know the change of plateletapheresis function in vitro by detecting the metabolic parameters of plateletapheresis during storage, and evaluate platelet quality roundly. 2, A modified method for buffy-coat-derived platelet concentrates and its qualityevaluationFirst centrifuge : 16 groups centrifuge conditions were applied to preparation of buffy-coat-derived platelet concentrates, and the platelet yields of four groups primary platelet products accorded with the national quality standards. The injury on the primary platelet products prepared through 2400 rpm(1861g) and 15 minutes was lowest, and the functional reservation of them was best.Second centrifuge: 9 groups centrifuge conditions were applied to preparation of ultimate platelet concentrates, and only the platelet yields, residual leukocyte and residual erythrocyte in 1100 rpm(391g) and 6minutes group completely accorded with the national quality standards. 3, Comparison of quality between buffy-coat-derived platelet concentrates andsingle-donor plateletapheresisThe platelet yields, residual leukocyte and residual erythrocyte in tow groups accorded with the national quality standard respectively, but residual leukocyte and residual erythrocyte of BC-PCs group were higher than them of SDPs group when platelet yields in tow groups were equal (P<0,01) . Lactate concentration, CD62p expression of platelet increased with prolongation of storage time,while pH value , decreased gradually.Compared with SDPs group, there were significant differences in CD62p expression, CD62p anew expression of platelet preserved for 0-5 days (P < 0.01), and in pH value of platelet preserved 2-5 days (P <0.01) .There was no changes in HSR of SDPs group for 0-5 days, while HSR of BC-PC group decreased gradually.Compared with SDPs group,there were significant differences in HSR of platelet preserved for 1-5 days (P<0.01) .It is concluded that the preparation technology of BC-PCs should be optimized ulteriorly in order to reduce residual leukocyte, residual erythrocyte and activated platelet yields, and improve the quality of BC-PCs.Based on these studies, the existing quality standard of platelet could not really reflect general quality level of platelet, and we should augment quality index reflectingmetabolism and the function of platelet in vitro in order to establish whole quality evaluation system on platelet, and apply reference for improving preparation technology of platelet.