Preparation Of Glycine Liposomes And It's Effect On Cardiomyocyte Injury Induced By Hypoxia/reoxygenation

AIM: To prepare glycine liposome microparticle and observe the effect of glycine liposomes on the release of enzyme from cardiomyocytes, the mitochondrial transmembrane potential and the apoptosis rate of cardiomyocytes induced by hypoxia/reoxygenation injury. This article provide new data and methods for the prevention and cure of ischemic heart disease.METHODS:(1)Reverse-phase evaporation method was used to formulate glycine liposomes,(1)the effects of different organic solvents: aether, chloroform and two mixtures of aether/chloroform on entrapment efficiency were evaluated; (2)Transmission electron microscope was used to detect the particle diameter of glycine liposomes; (3)Centrifugation was adopted to separate liposomes and not entraped glycine to evaluate the influence of different rotation speeds on the entrapment efficiency; (4)Glycine lipsomes was conserved at the condition of 4℃ to evaluate the stability of the entrapment efficiency.(2)A cardiomyocytes injury model was established by using hypoxia/reoxygenation, (1)the activities of lactate dehydrogenase(LDH), creatine kinase(CK) and creatine kinase isoenzyme(CK-MB) of each group were detected; (2)DiOC₆(3) as fluorescence molecular probe to detect the mitochondrial transmembrane potential of each group; (3)The method of Annexin V associate with PI was used to detect the apoptosis ratio of each group.RESULTS: (1)The entrapment efficiency of glycine liposomes prepared with the mixtures of aether/chloroform is highest compared with other organic solvents (64.8%, P<0.01); and there is no different effect of the three kinds of rotation speed on the entrapment efficiency (P>0.05).(2)A11 of glycine, glycine liposomes and blank liposomes inhibited the release of LDH CK and CK-MB induced by hypoxia/reoxygenation in cultured cardiomyocytes, and the inhibition of glycine liposomes was the most obvious (P<0.05, P<0.01). (3)The analysis result of flow cytometry show that the mitochondrial transmembrane potential of cardiomyocytes in H/R group is obviously lower than that of control...