| Objective: The aim of this study was to test in a large, randomized animal model, if Achilles tendon's Enthesiopathy may be prevented by local heat preconditioning and induction of heat shock protein 70(HSP70).Methods: In this experiment, 24 Wistar male rats were divided into the control group(n=4), the exercise group(n=9) and the preconditioning group(n=11) at random. Ankles of the rats from the preconditioning group were soaked in the warm water (about 42±0.5℃) for 15 minutes in advance. Then the rats from both preconditioning group and exercise group were stimulated by alternating current. They were killed after 4 and 8weeks respectively. The Achilles tendon terminal area were taken and carried on HE dyeing , induction of HSP70 were assessed quantitatively by immunohistochemistry.Result: Achilles tendon appear pathology change of the enthesiopathy in exercise group but not in preconditioning group; HSP70 can be measured both in exercise group and preconditioning group, but induction of HSP70 in preconditioning group was significantly higher than in exercise group .Conclusion and suggestion: Our results suggest that local heat preconditioning may be helpful for preventing the Achilles tendon's Enthesiopathy; the effect may be mediated by the induction of HSP70. Because local heat application is the most simple, cheapest, and least invasive method of preconditioning, we feel encouraged to evaluate this method in clinical trials.
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