| Objective①To study preparation and characterization of Mn-Zn ferrite magnetic nanoparticles②To study preparation and characterization of nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex③To evaluate the therapeutic effect of As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia (MFH) on the human cervival cancer cells④To investigate the therapeutic effect of As2O3/Mn0.5Zn0.5Fe2O4 complex combined with MFH on xenograft cervix cancer in nude mice.Methords①With the sulfate as the materials and NaOH as precipitator, Mn0.5Zn0.5Fe2O4 nanoparticles were prepared,which are characterizated by X-ray diffraction(XRD),transmission electron microscope(TEM) and heat analysis system .Besides, the heat effect was studied in alternating magnetic field in vitro②As2O3/Mn0.5Zn0.5Fe2O4 complex was prepared by impregnation process, and its properties were exmined through TEM,EDS and atom fluorescence spectrophotometer, drug releasing test in vitro③MTT assay was performed to examine the effect of nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex on the proliferation of Hela cells. By means of FCM, the ratio of apoptotic cells was detected.④After injected into xenograft human cervival cancer on nude mice respectively,Mn0.5Zn0.5Fe2O4 nanoparticles and As2O3/Mn0.5Zn0.5Fe2O4 complex were explored to AMF (SP-04C, 200kHz, 4kW, Shenzhen, China) for 30 min. The treatment was given for 3 times at 24h intervals. After 6 weeks, all mice were killed. the inhibition rate of the weight and volume of the tumors was calculated. At the same time, histological changes of tumors of tumor-bearing nude mice were observed under light microscope.⑤The levels of mRNA of HPVE7 was measured by RT-PCR.Results①the prepared Mn0.5Zn0.5Fe2O4 nanoparticles are spinel Mn-Zn ferrite ,global, average diameter 3040nm. The Mn0.5Zn0.5Fe2O4 nanoparticles have very distinct heat effect in a alternating magnetic field and can rise to a steady temperature for tumor hyperthermia (41℃～46℃).②The prepared As2O3/Mn0.5Zn0.5Fe2O4 complex are about global, average diameter 100nm; As2O3content is 0.61%.③Magnetic nano-microspheres containing As2O3 could significantly inhibit the proliferation of human Hela cells and promoted its apoptosis in vitro. The cell survival ratio in the group of nanosized As2O3/ Mn0.5Zn0.5Fe2O4 (41.69%) was distinctly lower than that of the As2O3solution (79.78% p<0.05) and that of the Mn0.5Zn0.5Fe2O4 nanoparticle magnetic fluid (59.48% p<0.05). The apoptosis ratio of magnetic nano-microspheres containing As2O3 (31.26%) was obviously higher than that of the other two groups (7.83%, 14.51%). The single magnetic material had no effect on cell growth in vitro④Animal experiment showed that the mass inhibitory ratio of nanosized As2O3/Mn0.5Zn0.5Fe2O4 was 96.70%, while As2O3 group and MFH group were 67.03%, 79.99% respectively. By means of SPSS11.5 software, the results showed that each group had distinct difference (p<0.01). On all accounts, the nanosized As2O3/Mn0.5Zn0.5Fe2O4 has both cytoxicity effects and the heating effects resulted from the radiofrequency-induced hyperthermia. And it has better effects on cervix cancer than either using As2O3 or hyperthermia alone.⑤The results of RT-PCR implies that 2μM As2O3 can inhibite the expression of HPV18-E7 oncogene, but this effect was not reinforced when combined with hyperthermia.Conclusions①Mn0.5Zn0.5Fe2O4nanoparticle and nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex could be prepared by improved co-precipitation process and impregnation process respectively.②Taking advantage of...
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