| Backgroud:Renal ischemia-reperfusion injury is one of the common reasons of the actue renal failure. There are still existing in actue renal injury, cardiopulmonary resuscitation, caidiopulmonary bypass and operation with renal artry occlusion. Renal ischemia-reperfusion injury is evitable pathophysiological process in kidney transplantation. Especially, along with the surgry of the kidney transplant was more and more maturer day after day, how to avoid renal ischemia-reperfusion injury in the transplant already become the hot spot in the domestic and foreign research. Sodium ferulate is extracted from the Chinese herbs, chuanqiong and angelica, which have a role of dilating blood vessel, inhibiting inflammatory reaction, anti-oxydation and cleaning free radical. It was reported that sodium ferulate could decrease the level of free radicals and increase the activities of antioxidant enzymes and protect the tissues from ischemia-reperfusion injury. Even though this medicine has been used in treatment of heart and brain diseases, there was no report on the use of it in treatment of renal ischaemia-reperfusion injury. In this study, we examined whether the sodium ferulate also play the same role against ROS following ischemia-reperfusion in rat kidney as in heart and brain. We observed that the activities of MDA and total SOD of organization were significantly enhanced in ischemia-reperfusion kidney in rat treated with sodium ferulate. We reported for the first time that sodium ferulate was useful in protecting kidney from ischemia-reperfusion injury in rat. Its clinical practice was also discussed. Objective: To investigate the protective effects and the mechanism of action of extract sodium ferulate, a model of post-ischemic renal injury by clamping renal pedicles for 45 minutes following 24 hour reperfusion was set up .Methods: Thirty-two male wistar rats (weighing 200-250g) were randomlygrouped into four groups : (1) normally group (2) sham group (3) I/R group (4) SF–pretreated I/R group. The plasma concentrations of creatinine and urea were measured for the assessment of renal dysfunction and injury. Besides, MDA levels and SOD activity were determined to assess lipid peroxidation. Pathologic changes were examined by optic and light microscope.Results: (1) The plasma concentrations of creatinine and urea in I/R group, higher than that both of the sham group and the normally group (p<0.01). SF–pretreated I/R group was shown to decrease the plasma concentrations of urea and creatinine respectively, there were significant differences compared with I/R group (p<0.01). however, there was no significant difference between the sham group and the normally group (p>0.05) .(2) In I/R group, MDA levels in renal cortex increased; whereas, SOD activity decreased, there were significant differences compared with the other group (p<0.01) .(3) Renal histopathology There were mass tuble epithelial cells edema, vacuolization, tuble dilatation, intraluminal casts and tuble necrosis in ischemia-reperfusion group compared with in sham-operated animals, these changes in SF group relieved.Conclusion: (1) SF pretreatment attenuates changes of SCr and BUN values, and impairments of renal tubule after renal ischemia-reperfusion injury in rat. These findings indicate that SF pretreatment has protective effects on renal ischemia-reperfusion injury in rats; the protective effect of SF might be due to its anti-oxygen free radicals and anti-peroxidation.
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