| The effect of Sodium ferulate on ischemiareperfusion jnjury in rat hearts in vivoObjectiveMyocardial ischemia-reperfusion injury is seriously pathophysiology process which occur after perfusion in acute myocardial infarction. Recently, with the development of all kinds of technology to treat acute myocardial infarction, myocardial ischemia-reperfusion injury has been paid more and more attention. People did a lot of research in basis and clinic to find a effective method which can prevent and treat m In this study we simulated clinical myocardial ischemia-reperfusion of acute myocardial infarction by making acute myocardial infarction model of wister rats to observe arrhythmic changes, content of malondialdehyde in the cardiac muscle and activity of Superoxide dismutase to reflect degree of injury cardiac muscle, we useD Sodium ferulate in ischemic period to interfere in myocardial ischemia-reperfusion injury in rat hearts in vivo to investigate the preventive and therapeutic effect of Sodium ferulate on myocardial ischemia-reperfusion injury in rat hearts in vivo.Materials and Methods40 wister rats are divided into four groups randomly, contrasting group, ischemia reperfusion group, Sodium ferulate large dose group and Sodium ferulate low dose group. Make model of myocardial ischemia- reperfusion of acute myocardial infarction, which the left anterior coronary descending artery was ligated in 10 min, then the ligateing was relieved 30 min. The incidence rate and the time of arrhythmia were recorded incessantly from myocardial ischemia to reperfusion. In the end and myocardial tissue of infracted zone was scissored to measure MDA content, SOD activity, iNOS proteinum concentration cNOS activity. Use x~2 test check groups compare the s and non-affair rate of the rat in different groups. When p＜0.05,ResultsThe occurrence rate of reperfusion arrhythemia (VF or VT) was 100% in ischemia reperfusion group, The time of appearing arrhythemia was obviously delayed, persistence time of arrhythemia was shorten, and incidence rate of ventricular tachycardia and ventricular fibrillation was reduced. MDA content was manifestly advanced in MI/R group; MDA content in Sodium ferulate low dose group was manifestly descended. There was significant difference (p＜0.05). There was more significant difference in large dose Sodium ferulate group (p＜0.01). On the contrast SOD activity in contrasting group was the highest in all groups. SOD activity in Sodium ferulate large dose group was manifestly lower than that of in MI/R group. There was significant difference (p＜0.05). iNOS activity in MI/R group was the highest in all groups, but cNOS activity was the lowest. All targets in Sodium ferulate 40mg/kg dose group were better than that of in Sodium ferulate 20mg/kg dose group.Conclusion1. Sodium ferulate has reduced arrhythemia on ischemia-reperfusion injury in rat hearts in vivo.2. Sodium ferulate eliminate free radical to protect myocard and improve cardiac function by diminishing MDA activity and enhancing SOD activity.3. Sodium ferulate has the effect of protection vascular endothelial cell and reversion systemic functional disorder of NOS.
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