Effect Of Inhibiting Nuclear Factor-κB Activity On Level Of Interleukin-8 In Retina Of Diabetic Rats

Objective: To investigate the effect of inhibiting nuclear factor-κB activity on level of interleukin-8 in retina of diabetic rats.Methods: The male Wistar rats were divided into 3 groups,group A(n=18) was the controls,group B(n=14) the diabetic rats without any therapy, and group C(n=11) the diabetic rats treated with pyrrolidine dithiocarbamate(PDTC,an inhibitor of NF-κB). The diabetic model was made by intraperitoneal injection of streptozotocin. At the end of the 16th week, the eyes were taken out from the rats to study the distribution and activity of NF-κBp65 by immunohistochemistry,meanwhile,IL-8 levels measured with an ELISA kit. In addition, The retina was then isolated from the optic disc and retinal trypsin digests were prepared. After PAS staining, the number of pericyte and epithelium cells and acelluar capillaries were counted in the cell image analysis system.Results: The activity of NF-κBp65 in group B(n=9,0.2513±0.1030 OD) increased and the average positive areas of NF-κBp65 were significantly higher than those in group A(n=12,0.1012±0.0210 OD,P<0.05),while the activity and distribution in group C(n=6,0.1427±0.0310 OD) lower than those in group B (P<0.05). Compared with that in group A(n=14,36±32pg/ml), the level of IL-8 in group B(n=9,373±336pg/ml,P<0.05) was remarkably high. The level of IL-8 in group C(n=9,146±137pg/ml) was remarkably lower than that in group B(P<0.05).The respective numbers of pericyte and acelluar capillaries were (6.72±0.42) and (3.50±3.12)in group A. Compared to those in group A,the number of pericytes decreased in group C(6.18±0.44) and more obviously in group B(5.57±0.59),on contrary, the number of acelluar capillaries increased in group C(16.30±3.89) and more obviously in group B(30.01±6.0).Conclusion : NF-κB is activated in the diabetic retinopathy.The inhibition of NF-κB can low down the development of DR and decrease the level of IL-8 in the diabetic retinopathy. Our data suggest that NF-κB may play an important role in the development of NF-κB.