Expression And Clinical Significance Of P53,Ki-67 And EGFR In 84 Cases Of Non-small Cell Lung Cancer

Background: Primary bronchogenic carcinoma which generated from bronchial mucosa or glandular is the most common cancer disease in the world, and it is also the main reason of cancer death. Recent years, with the development of molecular biology technology, people have achieved further understanding at the level of cells, genes, and the moleculars to cancer development. Some cancer-related genes and their products have been paid more and more attention to in cancer treatment. Thus, the study of cancer-related genes is of great significance. By clarifying the molecular events in the development of cancer, we can find which genes or products are most often involved in the carcinogenesis process, especially genes which are most often behaving default or inactivation, so that we can do some further study of these genes in their biological function and mechanism of transformation. On this basis, we can also design and adopt some targeting, meaningful, and effective treatment and ways of prevention. p53, a kind of anti-oncogene, has the function of regulating cell growth and their differentiation. It has been confirmed that expression of p53 has some relationship with the occurrence, development, invasion and metastasis in a variety of malignant tumors. Ki-67 is certainly a marker gene of nuclear proliferation, and also concerned with cell mitotic, And its product, ki-67 protein,is a kind of nuclear antigen associated with proliferating cell. EGFR is a kind of receptortyrosine kinase. Signal transduction mediated by EGFR has some relationship with the regulation of cell proliferation, survival, and differentiation. The abnormal expression and activation of EGFR often leads to the occurrence of cell transformation and tumor development. EGFR plays an important role in the occurance, development and metastasis of various of epithelial-derived tumors, including NSCLC.Objects: In this study, immunohistochemistry (S-P methods) is used to co-detect the expression of p53, ki-67 and EGFR in NSCLC. We attempt to recover the role of the 3 factors in tumor occurance and development, their relationship with tumor-associated factors, their relevance and clinical significance, so that they can provide us some relevant basis for clinical diagnosis and treatment of NSCLC.Method: Using immunohistochemistry (S-P methods) to co-detect the expression of p53, ki-67 and EGFR in 84 NSCLC lung tissue and 20 lung tissue of control group (normal lung tissue at 5cm distance to mass or another lobe from the same patients). X2 test and Spearman Correlation in SPSS for windows 15.0 are used in the study.Results:1. The expression of p53, ki-67 and EGFR are higher than those in normal tissue, P<0.05, it has significant difference in statistics .2. The positive expression rate (67.0%, 86.7%) of p53,Ki-67 in squamous cell carcinoma is higher than that in adenocarcinoma(66.7%, 85.5%), and, the positive expression rate of EGFR (63.3%) in squamous cell carcinoma is lower than that in adenocarcinoma (65%), but all the differences above are of no statistic significance. P>0.053. There is no significant difference in statistics of the 3 factor_s in the degree of tumor differentiation. The positive rate of ki-67 in the tumor diameter greater than or equal to 5cm group is 94.0%, it's higher than that in tumor diameter less than 5cm group. It has significant difference in Statistics. P<0.054. The positive rate of ki-67 and EGFR in the lymph node metastasis group are higher than that of the group without lymph node metastasis. The difference is statistically significant. P<0.05. The positive rate of p53 in the smoking index≥400ry group is higher than that in the group smoking index <400ry. The difference is statistically significant. P<0.055. The positive expression rate of p53,EGFR in stage I and II (60%, 54.5%)of NSCLC is lower than that of stage III and IV(82.8%, 79.3), the difference is statistically significant. P<0.056. Using Spearman correlation test, in the level p<0.01, the r_s between p53 and Ki-67 is 0.488, there is a positive correlation between p53 and ki-67.Using Spearman correlation test, in the level p<0.01, the r_s between ki-67 and EGFR is 0.488, there is a positive correlation between ki-67 and EGFR. The r_s between P53 and EGFR is 0.035, P>0.05, there is no correlation between p53 and EGFRConclusion:1. There is no expression of p53 and Ki-67 in normal lung tissue.2. Smoking may cause the occurance of NSCLC by forcing the p53 gene to behave deactivation and mutation .3. The high expression of ki-67 and EGFR in NSCLC can reflect the invasive and metastatic ability of the tumor cells.They can be used as indicators of the malignant degree to provide evidence about clinical diagnosis and the difficulty of surgery.4. Expression of p53, EGFR may provide an important reference for staging of NSCLC.5. The expression of Ki-67 reflects the tumor proliferative capacity, its expression can provide an important reference of whether or not to carry out neoadjuvant chemotherapy, radiotherapy, or chemotherapy.6. Ki-67 and EGFR are positively correlated,so as the p53 and ki-67, but there is no correlationship between p53 and EGFR. It suggested that ki-67 play an importmant role in the occurance and proliferation of NSCLC. There are synergies between the three factors. co-detecting and comprehensively assessing the three factors will be a good objective indicators in judging the degree of malignancy and development trends of NSCLC.