| AIM Due to high fatality rate and complex mechanisms, multiple organ dysfunction syndrome (MODS) is receiving increasing attention. Meanwhile, MODS is also a serious complication. Recently, MODS caused by brain injured has become the focus of research. The tea polyphenol (TP) belongs to phenolic compounds purified from tea leaves. The purpose of this thesis is to study the protective and TP actions of TP in MODS, which caused by cerebral ischemic reperfusion injury, further discussing its protective mechanisms.METHODS Adult male SD rats, of which the weight is 220±20g, were divided into 5 groups at random, and the 5 groups contained sham operation, model, TP50, TP100, and nimodipine control. The experiment adopted the improved blocking four arteries, that is, bilateral arteria carotis communis and bilateral arteria vertebralis, and to build the model of cerebral ischemic reperfusion injury(blocking 15min then reperfusion for 1h). Adm of TP was intravenous injection. Some indexes were observed, including the influence of TP with different doses on changes of water content in brain, the histomorphology of brain, lung and intestine. What is more, immunohistochemical indexes including HSP70 in brain tissue and HSP70 in lung tissue were observed as well.RESULTS1. The influence of TP on the changes of histomorphology in the model of cerebral ischemic reperfusion injury:1.1. Observed brain tissue by the microscope, sham operation , had clear cell layers, normal shape and gaps in cellular nervosa. When attention was drawn to model and TP groups, cellular swelling and dead cellular nervosas were indicated. Furthermore, other pathological changes have occurred such as karyopycnosis, gapsinflated, interstitial edema and so forth. TP groups were better than model in terms of pathological changes.1.2 Observed lung tissue by the microscope, angiotelectasis, thickened alveolar and exudation of akaryocytes could be observed in model and TP groups. TP groups were better than model in terms of pathological changes. Moreover, sham operation had no pathological change.1.3 Observed intestine tissue by the microscope, intestinal canals of sham operation had intact intestinal villus, which existed absorptive cells with columnar shape on surface. Furthermore, some lymph cells and plasma cells could be observed between fibrous tissues on lamina propria layer. And there was no edema and fibrosis on stratum submucosum and muscular layer.Some pathological changes, including disappeared intestinal villus, ablated cells, weak primary level and red cells on lamina propria layer could be observed in model.2. The influence of TP on the changes of water content in brain in the model of cerebral ischemic reperfusion injury: Compared with sham operation [(75.6±0.8)%], model [(78.8±0.6)%] significantly increased. Compared with model [(78.8±0.6)%], TP50 [(76.5±2.9)%] , TP100 [(77.2±0.9)%] and nimodipine control [(76.9±1.0)%] significantly decreased (P<0.01).3. The influence of TP on the changes of HSP70 expn in brain in the model of cerebral ischemic reperfusion injury:Compared with sham operation [(3.7±3.9)%], model [(54.6±14.1)%] significantly increased.Compared with model [(54.6±14.1)%], TP50 [(36.9±9.8)%] , TP100 [(19.5±9.8)%] and nimodipine control [(34.9±7.3)%] significantly decreased (P<0.01). Compared between TP50 [(36.9±9.8)%] and TP100 [(19.5±9.8)%], there was significant difference (P < 0.01). Meanwhile, dose-effect relationship could be concluded.4. The influence of TP on the changes of HSP70 expn in lung in the model of cerebral ischemic reperfusion injury:Compared with sham operation [(1.4±0.3)%], model [(39.2±7.9)%] significantly increased.Compared with model [(39.2±7.9)%], TP50 [(13.6±10.4)%] , TP100 [(4.2±0.3)%] and nimodipine control [(3.4±0.9)%] significantly decreased (P<0.01). Compared between TP50 [(13.6±10.4)%] and TP100 [(4.2±0.3)%], there wassignificant difference (P < 0. 01). Meanwhile, dose-effect relationship could be concluded.CONCLUSIONCerebral ischemic reperfusion injury caused brain injured, and also stimulates injury of lungs and intestinal injured. The TP relieved above injury, perhaps, there existed a relationship between mechanisms of anti-oxidation and declining stress reaction.
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