The Protective Effect Of Traditional Chinese Medicine Against Cerebral Ischemia-reperfusion Injury

Modern people are experiencing the change of their life model and thus cerebral ischemia attack has become one of the most lethal diseases threatening people's health. Ischemia-reperfusion injury in central nervous system often leads to neurological deficits, which seriously influence the quality of life. In 1990,Kitagawa et al firstly reported the induction of cerebral ischemic tolerance. Since then various researches have been done to find more substances which can induce ischemic tolerance. But few of their results can be clinically feasible. Acanthopanax senticosus, a traditional Chinese herb, and electroacupuncture,a new development of traditional acupuncture,are widely used in the treatment of cerebrovascular diseases. They were proved to be effective. This study was designed to explore the role that acanthopanax and preconditioning with electroacupuncture play in the protection of cerebral ischemia-reperfusion injury. This may give a new way to practicable measures for clinical use.-5-Objective1. To investigate the protective effect of acanthopanax senticosus against cerebral ischemia-reperfusion injury and its dose-response relation2. To investigate the protective effect of preconditioning with electroacupuncture against cerebral ischemia-reperfusion injury and its possible mechanismMethods1. Protective effects of acanthopanax senticosus injection on focal cerebral ischemia-reperfusion injury in rats and its dose-response relation40 male SD rats were randomly divided into 4 groups(n=10 each):Control group received no pharmacologic intervention but normal saline 7.5ml, Group AS -2.5, AS -5, AS -7.5 received intraperitoneally acanthopanax senticosus injection 2.5ml ^ 5ml (diluted to 7.5ml with normal saline)N 7.5ml respectively just before the right middle cerebral artery occlusion (MCAO) with 3-0 nylon monofilament for 120min. The neurological outcome was evaluated at 24h after reperfusion. The infarct volume was then assessed by TTC staining after the neurological outcome evaluation.2. Pretreatment with repeated electroacupuncture attenuates cerebral ischemia-reperfusion injury in rats-6-30 male SD rats were randomly divided into 3 groups(n=10 each) : Control group,animals received no treatment;Group ISO,animals inhaled 1.5% isoflurane 30 min a day for 5 days; Group EA,animals received electroacupuncture at the Baihui acupoint 30 min a day for 5 days under 1.5% isoflurane anesthesia. At 24h after last treatment,the right middle cerebral artery was occluded with 3-0 nylon monofilament for 120min. The neurological outcome was evaluated at 24h after reperfusion. The infarct volume was then assessed by TTC staining after the neurological outcome evaluation.3. Acupoint specificity of electropuncture's preconditioning effect on cerebral ischemic injury in rats40 male SD rats were randomly divided into 4 groups(n=10 each) : Control group,animals received no treatment; Group PB,animals were intraperitoneally injected with pentobarbitone 40mg/kg a day for 5 days; Group EL,animals received electroacupuncture at the left forelimb 30 min a day for 5 days under pentobarbitune anesthesia; Group EB,animals received electroacupuncture at the Baihui acupoint 30 min a day for 5 days under pentobarbitone anesthesia. 24h after the last treatment,the right middle cerebral artery was occluded with 3-0 nylon monofilament for 120min. The neurological outcome was evaluated at 24h after reperfusion. The infarct volume was then assessed by TTC staining after the neurological outcome evaluation.4. Pretreatment with repeated electropuncture increases HSP70-7-expression after cerebral ischemic injury in rats20 male SD rats were randomly divided into 2 groups(n=10 each) : Control group,animals received no treatment; Group E A,animals received electroacupuncture at the Baihui acupoint 30 min a day for 5 days. Then the right middle cerebral artery was occluded with 3-0 nylon monofilament for 120min. The pathological outcome and...