Empirical Study On Molecular Mechanism Of Mesangial Proliferative Nephritis Treated By Manshen NO.1 Prescription

Objective: By observing the change of the NO and the ET-1 contents in mouse's blood serum in each group including Normal control group,model group and treatment group to detect the molecular mechanism of the Manshen NO.1 prescription on restraining proliferation in intercaphillary cells and base material ,which can provide further theory evidence of curative effect for Manshen NO.1 prescription. As a result,Manshen NO.1 prescription can be applied more widely in clinical practiceMethods: Firstly ,we gave 40 mice fore-immunization by hypodermic injection CFA through two post- food pads;Secondly, intraperitoneal injection of BSA and i. v LPS through their tails were administered simultaneously for immunization; 4 weeks later, the MsPGN model was successfully made. 20 mice were chosen and divided into two groups at random with 10 mice in each group; besides, 10 mice were picked for normal control group. All the mice were administered via intragastric way per day for 4 weeks continuously. 0.5ml Manshen NO.1 prescription per mouse for treatment group, 0.5 ml NS per mouse for both Normal Control group and Model group. In the end, a comparison was made to detect 24 hours TUP ,renal function parameter, changes of NO and ET-1 contents , nephridial tissue examination.Results: 1,Compared with Normal Control group , NO contents decreases in Model Control(P〈0.05),but the ET-1 contents increased. After being treated with Nephritis NO.1 Prescription, NO and the ET-1 contents in Model group recovered(P〈0.05)2,Nephridial tissue examination indicated that there were lessened microscopic structural pathological changes ,cellular proliferation and widening mesangial region in mesangial cells through treatment compared to Model Control ,Conclusions :1 For Mesangial Proliferative Nephritis , Manshen NO.1Prescription has satisfactory therapeutic effects, It can improve symptoms, restrain accrementition in intercapillary cells and mesangial region, thus prevent nephric tubule's indurascent and prevent renal failure。2,Manshen NO.1 Prescription can increase NO contents; reduce ET-1 contents in Model control. 3,The reason Manshen NO.1 prescription can restrain accrementition in intercapillary cells and mesangial region may be due to its adjustment of NO and ET-1's contents in vivo. It is worth of doing further researchs in the future。...