The Therapeutic Effect Of The Intravenous Administration Of Bone Marrow Stromal Cells For Treating Transient Focal Cerebral Ischemia In Rats

Aim: To investigate the effect and mechanism of bone marrow stromal cells (MSCs) transplantation for treating cerebral ischemia in rats.Methods: Rat middle cerebral artery occlusion (MCAO) model was established. A total of 80 SD rats were divided into sham operation group,model group, PBS group and MSCs group at random. MSCs (2×106 ) labeled and the same volume of PBS were transferred into MSCs group and PBS group, respectively, through external carotid artery 24 h after stroke. Behavioral tests were performed before and after MCAO. Immunohistochemistry was used to identify MSCs and expression of neurotrophic factors.Results: The transferred MSCs survived,migrated and producing neurotrophic factors, such as VEGF after the transplantation. Compared with other three groups, the function of nervous system was improved.Conclusions: The therapy of MSCs transferring into rat brain after cerebral ischemia is effective. The neurotrophic factors produced by MSCs may explain the protective mechanism.