Study On Interactions Between Carbohydrates And Metal Ions & Detection Of Tumor Using FTIR

In this dissertation, erythritol and myo-inositol were chosen as model compounds of carbohydrates from chain alditol to ring inositol, and the interactions between carbohydrates and metal ions especially alkaline-earth metals and lanthanide ions were studied using FTIR(Fourier-Transform Infrared Spectroscopy), NMR(Nuclear Magnetic Resonance) , TGA-DTA (Thermal Gravity-Differential Thermal Analysis) and elemental analysis techniques.The comparison between the IR spectra of erythritol-Mg and alkaline-earth metals, transition-metals, lanthanide ions complexes indicate that the coordinated mode of Mg2+ is different from transition-metals and lanthanide ions; and same with one of the three Ca-erythritol complexes, Mg²⁺ can complex with erythritol in 2:1 proportion, each Mg ion is coordinated to three chloride ions, two water molecules and two OH group from one erythritol molecule.In contrast to chloride, the introduction of NO₃- in nitrate system is easy to form more coordination number complexes such as Ca(NO₃)₂ may form eight-fold complex with myo-inositol, and La(NO₃)₃ and Nd(NO₃)₃ may form similar ten-fold complex with myo-inositol. In FT-IR spectra CO stretching vibration bands (1200-950 cm^-1) represent different spectra appearances and peak positions corresponding to different crystal structures. The analytic result of NMR and TGA-DTA also provide the important proof of complex formation.The modified WQF-500 FT-IR Spectrometer from the Second Optical Instrumental Factory was used to detect the skin covering a parotid gland using an accessory (SpecTech)of mid-IR fiber optics with an ATR probe. In this investigation, forty patients with tumor of salivary gland were performed using FT-IR fiber optics measurement. The differential diagnosis from the obtained FT-IR spectra of benign and malignant tumors of salivary gland was compared with pathological diagnosis, which is considered as the standard. Good agreements were found between the results of pathological diagnosis and those of spectral detection. The results showed that the difference lied in the obtained FT-IR spectra of the skin covering benign and malignant tumors of salivary gland and normal parotid gland. And the difference can be characterized by the band width, peak position and peak intensity. Based on the difference of the band in the range of 1500¹000cm^-1, malignant tumor can be classified into two types. The first type includes acinic cell carcinoma, oxyphilic cell adenocarcinoma , adenoid cystic carcinoma and so on. Along with the variation of species (for example malignant lymphoma, lympho-epithelial carcinoma and so on), great changes take place in band width, peak position and relative intensity of the band between 1500cm^-1 and 1000cm^-1 in spectra of the other kind of malignant tumor compared to the first type. So the FT-mid-IR spectroscopy is a promising technique to become a non-invasive, quick, accurate and convenient method to diagnose malignant and benign tumor of salivary gland early.