Inducement Of Dendritic Cells Derived From Cord Blood With GPS And Identification Of DCs

Objective The present study explored the impact of the inducement culture of dendritic cells from cord blood using Ginseng Polydsaocharides(GPS). Methods Cord blood was collected under steril condition and mononuclear cells were isolated from cord blood by Ficoll-Hypaque density gradient centrifugation.These cells were cultured in 12-well culture plates with RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum.After three hours of culture nonadherant cells were gently removed and fresh medium was added into the culture in the presence of GPS(0μg/rnl,50μg/ml,100μg/ml,200μg/ml respectively) and combination of cytokines (GM-CSF/IL-4/TNF-α).Cultured cells were analyzed by microscope and numbers of lived cells were calculated by trypan blue. Cultured cells labeled with monoclonal antibodies by immunology fluorescene were detected by flow cytometry to find CD80,CD83,CD86 cells positive rate on 12th. Results After culture of GPS and cytokines,the cells appear typical morphotype,the survive numbers of cultured cells were enhanced markedly and CD80,CD83,CD86 of DCs were significantly highly expressed. Conclusions Our findings suggested that cord blood mononuclear cells can be induced into DCs with GPS and cytokines.The study can be conformed by the morphologic feature,phenotypic expression.The research has the important clinical and research value to search a new method of generating DCs.