| Objective: In order to study the action ofα-amylase inhibition which were separated and purified from wheat by biochemical center. In this topic, we study the action ofα-amylase inhibition in hypoglycemic,weight-reducing,hyperlipemia and its mechanism. It provides references forα-amylase inhibition developed as a new health product or drug.Methods:(1) The hypoglycemic action ofα-amylase inhibition: Diabetes mice were treated withα-amylase inhibition(5,15,45 mg·kg-1) by gastric perfusion for 21d and their BG level and disaccharidase activity were measured. Diabetes mice were treated with AI 45mg/kg by gastric perfusion and their starch and sugar tolerance were measured. Disaccharidase activity in various segments of mice'small intestine was determined by Dahlqvist method.(2) The hyperlipemia action ofα-amylase inhibition: Use different dose ofα-amylase inhibition treat the hemorheology rat in the prevention study and therapeutic study. The concentration of TC,TG,HDL-C,LDL-C in serum and TC,TG in liver was determined, and the hemorheology and pathological changes of liver and artery were also observed.(3) The weight-reducing action ofα-amylase inhibition: Fat rat were treated withα-amylase inhibition(5,15,45 mg·kg-1) by gastric perfusion for 28d and their weight,lees and fat's weight were measured.Result:(1)α-amylase inhibition can increase the starch tolerance of diabetes mice but not change the sugar tolerance. The BG level in diabetes mice received 15 and 45 mg·kg-1 ofα-amylase inhibition for 21d was significantly decreased in comparison with the model control group.α-amylase inhibition also could inhibit the disaccharidase activity in various segments of mice small intestine.(2) In the prevention study,α-amylase inhibitor of the middle and high doses can markedly decrease the concentration of TC,TG,LDL-C in serum, TC,TG in liver,...
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