ERIC-PCR Fingerprinting Of Structural Features Of Microbial Communities In Uygur People's Intestines With Different Types Of Blood Glucose

Objective: To characterize the structural features of intestinal communities in xinjiang Uygur people's fecal samples of different types of blood glucose by ERIC-PCR fingerprinting.And to study the relationship of intestinal microflora and type 2 diabetes mellitus. Methods: 40 normal fasting blood sugar(NFBG) people,11 type 2 diabetes mellitus (T2DM) ones and 3 impaired glucose tolerance(IGT) ones were selected according to diagnostic criteria of WHO in 1999.The genomic DNA of bacteria was extracted from each sample, ERIC-PCR amplification was then carried out and thus obtaining DNA fingerprints. Results: The banding-patterns of different samples were bright and foreign material of DNA was little. The DNA fingerprints of different samples from the same individuals were highly similar. However, a few strong signals were commonly found in the fingerprints of samples from NFBG people, but no or weak signals were found in these from T2DM and IGT ones. Conclusion: The method of total DNA extraction was reliable and convenient. The experiment condition of ERIC-PCR was stable. The structure of intestinal microflora might be closely related to T2DM.