| Ischemic heart disease is caused by coronal blood circulation changes and induces the imbalance of myocardial supply and demand. its most common pathogenesis is the straightening and obstruction of the coronary arteries from atherosclerosis. Whether in developed countries or in developing countries, IHD is a deadly and deforming disease .so widely epidemic that it imperils the whole of humankind's health. The therapeutic arsenal that is currently available to physicians in combating IHD targets is deficient in direct cardio protective agents. A safe and effective new therapy that shows the progression of myocardial ischemic injury and protects the myocardial cell would therefore be expected to synergies with existing therapies and provide considerable benefit.Re,a monomer of panaxadiol saponins,has been proved that it can decrease the heart rate, myocardial oxygen consumption, coronary arterial resistance. Re could ameliorate the index of hemodynamics, decrease the raised myocardial enzyme, the positive percent of cardiocytes apoptosis and reduce the infarction area. All of this we stated above showed that Re have the effect of protect the cardiocytes. In order to investigate the direct function of Re on ischemic cardiocytes and to provide theoretical reason for the clinical, we obtained single active ca2+ tolerance guinea pig ventricular myocytes by acute enzymatic dissociation. L-type calcium channel current (IL-ca), adenosine triphosphate-sensitive potassium channel current (IKATP) and action potential were recorded using the whole cell patch-clamp technique in ischemic cardiocytes.1. Isolation of ca2+ tolerant cardiocytes...
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