The Harm Of Nitrobenzene On The Liver And Heart In Rats And The Effect Of Vitamin C Antagonize Nitrobenzene

Objective:To investigate the influence of nitrobenzene on the blood system,heart(the mechanics index of cardiac muscle and the biochemistry trait of tissue)and liver (the biochemistry trait of tissue) ,to explore if the vitamin C antagonize nitrobenzene in rats.method: In this rat experiments, we measure the changes in myocardial mechanics, biochemistry in blood and tissues. The mechanics index of cardiac muscle include left ventricle shrink pressure ,left ventricle end shrink pressure,+dp/dt,-dp/dt. The index about biochemistry include the activity of superoxide dismutase of heart and liver,the content of malondialdehyde of heart and liver in rats. The index of blood includes mean artery pressure and volume of erythrocyte.Results: 1.Comparing to the rats in the normal and dimethylsulfoxide group, the mechanics index of cardiac muscle were lower with the increased dose of nitrobenzene,-dp/dt changed observably in 15mg/kg nitrobenzene group(comparing with normal group, it decrease 40.53%), the other indexes changed observably in 45mg/kg nitrobenzene group.( comparing with normal group, left ventricle shrink pressure, left ventricle end diastolic pressure,+ dp/dt decrease,27.48%,77.94,63.78% respectively) 45mg/kg。2.The number of red blood cell reduced rapidly after injecting nitrobenzene , It decreased 8.39% in 15mg/kg nitrobenzene group. the more nitrobenzene ,the less the number of red blood cell. Artery mean pressure decreased distinctly in 45mg/kg nitrobenzene group (comparing with normal group, it decrease 38.5%) ,after this, the artery mean pressure were much lower with the increased dose of nitrobenzene.3. The content of malondialdehyde of heart and liver in rats increased evidently in 15mg/kg nitrobenzene group(it increased 74.74% and 125.03% in heart and liver respectively).when the dose of nitrobenzene increased, the content of malondialdehyde enhanced .the activity of superoxide dismutase of heart and liver increased little in 15mg/kg nitrobenzene group, when the dose of nitrobenzene increased, the activity of superoxide dismutase reduced evidently (in 45mg/kg nitrobenzene group, the activity of superoxide dismutase of heart and liver decreased 49.54% and 67.45% respectively). The more dose of nitrobenzene, the less activity of superoxide dismutase and the more content of malondialdehyde. 4. In the vitamin C against nitrobenzene group, the activity of superoxide dismutase of heart increased (it increased 11.56%), the other indexes had no distinct. change. Conclusion: nitrobenzene had obvious influences on the mechanics of cardiac muscle, the number of erythrocyte, the activity of superoxide dismutase of heart and liver, the content of malondialdehyde of heart and liver in rats. the vitamin C can enhanced the activity of superoxide dismutase of heart, but have no effect to the other indexes.