| Fundamental study in free radical iatrology showed that oxygen radicals relate to hundreds of diseases. In the last 20years, active oxygen and free radical have become the hot topics of modern life science, and selection of natural resources with strong antioxidative activities have become the new trends of biology, medical science and food science and technology. Antioxidants in Chinese traditional medicine may be useful in vivo and can be used to prevent oxidation impair. So more and more researchs focus on it.The root tuber of the Cynanchum auriculatum Royle ex Wight of the genus of Cynanchum is the main origin of the Baishouwu in Binhai county. As a traditional Chinese medicine, Baishouwu has been used in Jiangsu province for hundreds years.It has been reported that Baishouwu exhibited several potent pharmacological activities, including anti-tumor, antioxidant ability and immunologic enhancement. In this paper, optimum extract conditions of antioxidant from Baishouwu in Binhai county and purifucation conditions of absorbent resin column chromatography were studied, and the antioxidant activity were evaluated using vivo test.: Based on the results of one-factor-at-a-time-technique, a three-level Box-Behnken factorial design combining with response surface methodology(RSM) was employed to optimize the extraction condition of Baishouwu. When with two-time extraction, the extraction conditions of temperature, time and concentration of ethanol were set at 70.1℃,1.78 h and 75%,respectively. The activities of oxidation resistance of the ethanol extracts on the extraction condition of optimization were measured by lard, soybean oil and peanut oil as substrates. POV were measured by Oven Storage Method, and the synergistic actions were also investigated with viamin C and citric acid. The result showed that the optimum ethanol extracts have antioxidative activities to resist lipin-oxidation. Viamin C had synergistic antioxidative activities with ethanol extracts, but the synergistic effect of citric acid were not very significant.By comparing static and dynamic absorption performance, macroreticular resin HPD400 that had highest performance in absorbing antioxidative extract of Baishouwu and optimum absorption relieving agent 80% ethanol solution were selected. After absorption and purification by HPD400, the amount of total phenolic and total flavones compounds were increased by 10.18, 2.53 times. phenolic compounds, steroidal glycoside, and flavones in extract were confirmed by chemical qualitative analysis. Antioxidant activities of ethanol extract of macroreticular resin of Baishouwu(EEOMR) were evaluated using different in vitro tests: the DPPH radical scavenging activity, the reducing power, the inhibitory effect on hydroxyl radical and superoxide anion radical, the inhibitory effect on oxidation of linoleic acid. EEOMR was found to have antioxidant activities in all the model systems tested except the model of superoxide anion radical .The senior modle of Kunming-mice was built with D-galactose. Different doses of EEOMR were administered to the experimental mice. brain index, heart index, liver index, spleen index, kidney index and thymus index were measured. MDA and SOD of mice serum, brain, heart, liver and GSH-Px of mice liver, were measured. The results indicated that EEOMR could (1) effect on spleen index of mice (P<0.05), (2) increase the activities of SOD(P<0.01), reduce the level of MDA in heart, (3) reduce the level of MDA in brain (4) in crease the activities of SOD(P<0.05), GSH-Px/LPO ratio(P<0.01) and reduce the level of MDA in liver, (5) increase the activities of SOD(P<0.05), reduce the lever of MDA in serum.
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