Study On The Extracellular Proteinase Of Malassizia Furfur

Objective: ①To study the extracellular proteinase activity of M. furfur; ②To study the difference between mycelial phase and yeast phase in the proteinase activity;③To study the effect factors of proteinase activity.Methods: The standard strain of M. furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M. furfur. At the same time, selected the culture supernatant. Used the whole-fat-cow's-milk plate method, the Folin-phenol method and the QuantiCleave proteinase Assay Kit to test the proteinase activity of mycelial phase and yeast phase of M. furfur. The effect of temperature and pH on this assay was also evaluated.Result: ①The whole-fat-cow's-milk plate method: Seven days after inoculation, the proteinase activity of the mycelial phase supernatant (12.5 ± 1.2mm) is higher than that of the yeast phase (9.25±0.71mm) (p<0.05); With the passage of the inoculation time, the proteinase activity of the mycelial phase is rising; the temperature has no obvious effect on the proteinase...