| [Objective] : To study the effects of epidermal growth factor on the development of two-cell mouse embryos in vitro, investigate the mechanism of embryogenesis and optimize the culture medium for preimplantation embryos. To provide the theory and experimental bases for the optimization of the culture medium of mankind embryos.[Methods] : Tow-cell embryos were divided randomly and cultured in vitro in five groups: [1] control: mKSOM medium with no EGF as groupl; [2] Treatments: 4 groups, mKSOM medium with 0.1, 1, 10 and 100ng/ml EGF respectively as group 2, 3, 4, 5. Blastocyst rates hatched blastocyst rates and the blastocyst's cell numbers were counted during 96 hours cultured period. [Results]: 726 two-cell embryos were collected and cultured up to 96 hours. Results: [1] at the time point of 72h culture, blastocyst rates were 93.8%, 92.4%, 91.8% and 91.2% respectively for group2, 3, 4, 5. The rates were significantly higher than that of the control (79.9%, P<0.05); but there were no significant (P>0.05) differences of blastocyst rates among group2, 3, 4, 5. [2] at the time point of 96h culture, rates of hateched blastocyst were 61.1%, 61.8%, 63.0% and 60.1% respectively for group 2, 3, 4, 5; The rates were significantly higher than that of the control (43.1%, P<0.05); but there were no significant (P>0.05) differences of hatched blastocyst rates among group 2, 3, 4, 5. [3] at the time point of 96h culture, the cell numbers of blastocyst were 72.1+6.9, 73.1 + 13.4, 73.8+7.0, 74.3+12.2 and 77.6+8.2 respectively for the five groups; There were no significant (P>0.05) differences among them. [Conclusion] : Among our study range of the concentrations 0.1~100ng/ml: EGF [1] could increase blastocyst rates of two-cell mouse embryos cultured in vitro. [2] could improve hatched blastocyst rates of the embryos. [3] has no effect on cell numbers of blastocyst. [4] has no toxic effect on...
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