A Clinical Study Of The Renal & Intestinal Injury At The Anhepatic Phase Of Non-bypass Liver Transplantation Operation

PurposeThe purpose of the present thesis is to observe the functional injury to kidney and small intestine that may occur during the surgical operation of liver transplantation without venovenous bypass.MethodThe method of clinical self-control experiment is applied for this study. During the surgical operation of the liver transplantation without venovenous bypass, a sample of peripheral vein blood (5ml) is collected for testing the content of serum urea nitrogen(BUN), blood serum creatinine(Cr), Cystatin C, and plasma endotoxin (LPS). In the meantime, a sample of urine (5ml) is also collected for testing the content of urease(NGA) and micro-amount of urine albumin(UALB). Blood and urine samples are collected at ten different phases of liver transplantation: removal of the diseased liver, 40 minutes after the removal of the diseased liver (or anhepatic phase), and 10 minutes, 1h, 3h, 6h, 12h, 24h, 4Sh, 72h respectively after the transplanted liver has started reflow. Through analytical comparison of the testing results of the samples collected during the anhepatic phase (40 minutes) and after reflowing of the newly transplanted liver with those of the samples collected during the time when the diseased liver is being removed, we can learn the degree of injury that may occur to the patient's kidney and small intestine during the process of surgical operation of liver transplantation without venovenous bypass.ResultOn comparing the testing results of different phases, we have learnt that the content indexes of blood and urine increase obviously (P<0.01 = at anhepatic phase (40 minutes) and at reflow phase of the newly transplanted liver. The content of blood...