The Clinlcal Significance Of Detecting IgH By Real-Time Quantitative PCR In B-cell Lymphomes

Objective To investigate the clinical value immunoglobulin of heavy chain(IgH) gene rearrangements in the diagnosis and follow-up of B-cell Lymphomes.Methods Real-time PCR was used to quantitive the IgH rearrangements.Specimens included paraffin-embedded tissue sections from 15 cases of B-NHL. Paraffin-embedded tissue sections from 2 cases of T-NHL and 3 chronic lymphadenitis were selected as control group.Follow-Up monitoring of the numbers of copies of IgH of peripheral blood and bone marrow smears which were obtained simultaneously from 10 patients with B-NHL after chemotherapy.Results It was found that numbers of copies of IgH rearranements were detected inl2 cases of B-NHL , however,3 cases of B-NHL were not investigated the numbers. 5 control cases were found not to investigate the numbers of copies of IgH rearranements. The numbers were detected in the 10 patients with peripheral blood and bone marrow smears obtained simultaneously. Furthermore there were no statistical difference between them. (P=0.343 > 0.05)Conclusion The quantitative analysis of IgH rearrangement by real-time PCR can be an adjuvant tool for diagnosis of B-NHL and detection of minimal residual disease. It may play an important role in evaluating the chemotherapy efficiency and predicting relapse. It was no statistical difference for the numbers of copies of IgH rearranement between peripheral blood and bone marrow smears. The quantitative assessment of IgH rearrangement in peripheral blood by RQ-PCR may be a useful tool for monitoring minimal residual disease( MRD).