Research On Tyrosine Phosphorylation Of Leptin Receptors In Experimental Fatty Liver And Hepatic Cirrhosis

Objective: To explore the changes of Leptin recepors tyrosine phosphorylation in experimental fatty liver and hepatic cirrhosis. And to know the molecular biologic mechanisms of fatty liver and hepatic cirrhosis by comparing the relations of different level of protein expression and tyrosine phosphorylation respectivly. And to discuss the possible effects and correlation of leptin in the development of fatty liver and liver fibrosis by ways of cell signal transduction. So provide experimental bases for the patho-genesis and development of fatty liver and liver cirrhosis.Methods: The expeimental rats were divide into fatty liver group(n=20) and hepatic cirrhosis group(n=13).One control group was used(n=10).fatty liver model were duplicated by high fat diet feeding and hepatic cirrhosis, peritoneal injection of thioacetamide(TAA). The rats'stem lenth, weight were measured during the raising period. The hepatic tissues were sliced for pathological observation. Tyrosine phosphorylation of leptin receptors was measured by Co-immunoprecipitation and Enhanced Chemiluminescence (ECL) technique. The outcomes were analysised quantitativly by image analysis software.Results:fatty liver and hepatic cirrhosis models were successfully induced. The OD value of of leptin receptor in rat hepatic tissu was significantly decreased in fatty group compared with the hepatic cirrhosis group and the control(P < 0.01). Nevertheless, compared with the two groups of hepatic cirrhosis and control,there was no difference of the OD value of protein tyrosine phosphorylation of leptin receptor( P > 0.05) .Conclusions: 1. The decreasing of the level of protein tyrosine...