The Cloning And Expression Of HE4 Gene And Its Preparation Of McAbs

Ovarian cancer has high dead rate and is hardly found in the early stage, so it is thought to be one of the most deadly cancer. Cancer is potentially curable if diagnosed when localized, but most ovarian cancers are not diagnosed at an advanced stage. The survival rate is <20% despite aggressive surgery and chemotherapy.Currently, the only clinically accepted serum marker for ovarian cancer is CA125, which is found elevated in the majority of all ovarian cancers but in only half of those with early stage. Although overall CA125 has high sensitivity, its clinical use as an early detection marker for screening is severely limited, because it is also frequently elevated in women with more highly prevalent benign disease. Because these false positives are not easily eliminated by available imaging technologies, CA125-based screening would result in a large number of unnecessary surgeries. There is a need for novel biomarker that is sensitive and specific to improve diagnosis.HE4 gene is a novel biomarker for ovarian carcinomas in combination with CA125 or that can replace it. Schumme found HE4 was amplified in ovarian carcinomas but not in normal tissues. HE4 belongs to a "four-disulfide core" family. Some members of the four-disulfide core family of proteins are protease inhibitors. No protease inhibitory activity has been identified for HE4, whose function remains...