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Experimental Studies On TIMP-1 And A-Actin Of Injuried Heart Caused By Electric Current

Posted on:2007-12-15Degree:MasterType:Thesis
Country:ChinaCandidate:S T PanFull Text:PDF
GTID:2144360185988231Subject:Forensic medicine
Abstract/Summary:PDF Full Text Request
Objective In recent years, electrical injury is more year by year, In forensic medicine, the cases of murder and suicide are not unsusal. Forensic scientises in domestic and overseas can't betterly diagnosy posthumous electric injury,electric injury after death ,even markers of injury time. For those aims,we desigened this experimental study on electrical injury of heart.Methods 1. The models of injured heart caused by electric current were replicated on wistar rats by current(220V,50Hz,150-200mA) for 30 seconds. 2. We excuted theses rats by decapitation and took out their heart tissue at different time (0min 30min, 1h, 2h, 3h, 4h,5h,6h, 8h, 12h, 1d, 3d, 6d and 12d);We excuted normal wistar rats by decapitation and replicated on rat models(0min,30min and 2h,the same to mod).then stored respectively the heart into 10% formalin for regular petrolin slice, the slices were stained by HE,immunohistochemistry for lighting microscope observation.Results: From 0 min to 6 hours, myocardial cells focal degeneration and...
Keywords/Search Tags:heart, electrical injury, TIMP-1, a-Actin
PDF Full Text Request
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