Myocardium Ultrastructural Changes And The Expression Of TNF-α In The Myocardium Of Different Impaired Glucose Metabolism Rats

Purpose: (1)To observe the changes of the metabolic in different impaired glucose metabolism subcategories rats .(2) To observe the changes of the morphology of myocardial structure and myocardium ultrastructural in different impaired glucose metabolism subcategories rats .(3) To observe the changes of the the expression of TNF-αin the myocardium of different impaired glucose metabolism subcategories rats . (4)To investigate the relationship between the metabolic alteration and diabetic cardiomypathy, to investigate the inflammatory mechanism of development of diabetic cardiomypathy.Method: 40 Wistar rats(specified-pathogens free) of 4 months old in male randomly divided into the 4 groups: A, B, C, D and each one is 10. Among them A is the normal control group, feeding with regular diets, 4 weeks later injects citrate buffer liquid in obdominal cavity; B is the insulin resistance group, feeding with the diets enriched with high-sucrose and high-lipid, 4 weeks later injects citrate buffer liquid in obdominal cavity; C is the type 2 diabetes with insulin resistance group, feeding with the diets enriched with high-sucrose and high-lipid, 4 weeks later injects the aqua of streptozotocin (STZ) by the 30 mg/ kg weight measures; D is the type 2 diabetes group with insulin secretion defaulting, feeding with regular diets, after 4 weeks later injects the aqua of STZ by the 50mg/kg weight measures. The expression of TNF-αwere determined by...