Genetic Analysis Of Haplotypes In Apolipoprotein A1-C3 Gene Region: Effects On Coronary Heart Disease And Type 2 Diabetes

Objective: Many epidemiological studies or reviews have demonstrated that dyslipidemia was associated with heterogeneity of the onset and severity of coronary heart disease (CHD), in addition, it is also a clinical feature of type 2 diabetes mellitus (T2DM), and then it has been proposed to be a risk factor for T2DM. The purpose of this study was to evaluate the influence of variants in the lipid metabolism related genes——apolipoprotein( APO) A1, C3 on the risk of developing CHD and T2DM. These variants were -75G/A and +83C/T polymorphisms in the APOA1 gene, -482C/T and -455T/C polymorphisms in the APOC3 gene.Methods: we genotyped these four single nucleotide polymorphisms (SNP) in 310 patients with CHD,453 patients with T2DM and in 499 health free of CHD and T2DM. Then 4 SNP haplotypes and 2 SNP haplotypes were constructed for the association study. Case-control comparisons were performed separately for CHD and T2DM by using SPSS statistical software package.Results: Single marker analysis demonstrated that none of the four univariants was related to CHD risk. However, the case-control studies showed that -482TT ,TC genotype and -455TT genotype were associated with protective effects against T2DM with odds ratios (OR) 0.35,0.63 and 0.36 respectively. And haplotype analysis...