Relationship Between Caspase-8 And Fhit Gene Expression In Bladder Neoplasm

0BJETIVE To study the role of Caspase-8, Fhit in the development of carcinoma of bladder, as well as its clinical significance, and to investigate the relationship between Caspase-8 and Fhit protein expression. METHODS The expression of Caspase-8 and Fhit were measured by immunohistochemistry in the normal bladder samples, cystitis glandularis> paraneoplastic samples and carcinoma of bladder, and their average optical by image analysis system.RESULTS The average optical of Caspase-8 and Fhit in normal bladder samples, cystitis glandularis, paraneoplastic samples, transitional cell carcinoma of bladder and adenocarcinoma of bladder was respectively (0.248±0.044),(0.270 ±0.042); (0.150 + 0.051),(0.142±0.040); (0.234 + 0.053), (0.240 + 0.061); (0.108 ±0.053), (0.097 ±0.053); (0.105 ±0.062), (0.089 ±0.026) .Successively the expression of Caspase-8 and Fhit in carcinoma of bladder was significantly lower than that in normal bladder samples (P<0. 01) . The intensity of Fhit and Caspase-8 expression was decreased with the increasing of grades of tumor (P<0.01) , and obviously correlated with the grades respectively (r= - 0. 674; P<0. 01), (r= - 0. 700; P<0.01) , but incorrelated with stages of tumor (P>0. 05) . A positive correlation was found between Fhit protein loss and Caspase-8 reduced expression (r=0. 726; P<0.01) .CONCLUSION The down regulation of Caspase-8 expression in bladder neoplasm in connection with Fhit protein loss reveals the close relationship between Caspase-8, Fhit and the occurrence and progression of the cancer.