Research Of Related-Genes Of Non-Hodgkin's Lymphoma

Non-Hodgkin's Lymphoma(NHL) is one kind of heterogeneous diseases, and the expression of related-genes plays an important role in occurrence, development, diagnosis, therapy, and prognosis of NHL. The study of gene expression of NHL can further reveal the biologic nature underlying the morphology and the clinical behavior.Firstly, a gene expression profiling of 116 clones is prepared and used to detect the gene expression profiles of the lineage cells of BL,CTCL and normal lymphocytes. 6 up-regulated and 4 down-regulated genes are identified in BL lineage cells, and 6 up-regulated and 2 down-regulated genes are identified in CTCL lineage cells. Secondly, the results are identified and verified by semi-quantitative RT-PCR. On this foundation, 9 genes(c-myc, bcl-2, bcl-6, survivn, casp10, pax5, junb, riz,k-ras)are picked and expression of these genes in 25 tumor tissue samples of NHL patients and 5 lymphatic tissue samples of chronic lymphadenitis is detected by RT-PCR. Finally, the relationship between the alteration of gene expression and pathological subtype, clinical behavior, response to treatment and prognosis of NHL is discussed on the basis of the RT-PCR results of samples.Genes expression is found to be different in distinct subtypes. High expression of c-myc, bcl-2 and survivn has adverse impacts, whereas high expression of bcl-6, casp10 has favorable impacts on prognosis of high-grade B-NHL by the survival analysis. The alteration expression of c-myc, bcl-2, bcl-6, survivin and casplO is also found to have correlation to the therapeutic effect of B-NHL.