Study Of A New Tumor Marker EpCAM Expressed In Gastric Cancer And Clinical Value

Background: The mortality of gastric carcinoma, one of the familiar malignant tumor, stands first on the list. It is important to detect and diagnose in nonage in order to prevent and cure gastric carcinoma. Study of tumor marker will improve it. EpCAM, a surface adhesion molecule expressed on epithelial cells and overexpressed on malignant epithelial cells such as lung cancer, colon cancer, rectum cancer, pancreas cancer, et al, is a new pan-carcinoma antigen. Tissue microarray(TMA) are a powerful new technology for high throughput analysis of biomarker expression in a large number of tissue samples. Tissue microarrays have numerous advantages over regular sections: speed, throughput, standardization, ease-of-use. conservation of valuable tissue. It may have a powerful value to promote the development of medicinal molecule biology.Objective: To study a new tumor marker EpCAM expressed in 91 cases of gastric cancer and clinical value by tissue microarray, and explore the feasibility of EpCAM as a tumor maker of gastric cancer. This study was to help diagnosing gastric cancer.Methods: A total of 91 primary gastric cancer and 79 normal tissue specimens were obtained from gastric cancer patients undergoing surgical therapy for the treatment of gastric cancer. At least two core tissue biopsies 1mm in diameter were taken from selected morphologically representative regions of each paraffin-embedded gastric cancer and precisely arrayed using a custom-built instrument. Additional core tissue biopsies were taken from morphologically normal tissue. All we got three three-um-thick sections of the tissue microarrays block. EpCAM protein expression in tumors and matched morphologically normal gastric tissues was evaluated using anti- EpCAM immunohistochemistry. The relationship of EpCAM protein expression in tumors tissue and normal tissue was analyzed. And the difference of EpCAM protein expression in...