| Objective: To analyse the expression of vascular endothelial growth factor D (VEGF-D),microlymphatic density (MLD) and microvessel density (MVD) in middle and low rectal cancer.And to discuss the significance of lymphangiogenesis in the development of rectal cancer ,lymph node metastasis and its association with the recurrence of the post operation of sphincter saving in middle and low rectal cancer. Method: We gathered 80 specimens of middle and low cancer who underwent rectectomy in the First Affiliated Hospital of Anhui Medical University. And the follow-up data of these cases are integrate.40 specimens biopsied from rectal polyps and 80 normal rectal tissue were examined as control.Immunohistochemical technique was used to examine the expressions of VEGF-D,MLD and MVD.Their relationships with tumor size, gross morphology,histologic type, metastasis, differentiation grade, infiltration depth , Dukes stage, lymph node metastasis, prognosis and the recurrence of the post operation of sphincter saving were also dicussed.Result: 1.The positive rate of VEGF-D was 55% in rectal cancer ,0 in rectal polyps and 0 in normal rectal tissue. The expression of VEGF-D in rectal cancer was higher in rectal polyps and normal rectal tissue (P<0.05) . While MLD was 2.80±1.306 in rectal cancer (The Media is 3.3,the maximum is 7.3, the minimum is1.0) ,0.50 ± 0.716 in rectal polyps (The Media is 0.00,the maximum is2.7, the minimum is 0) and 0.25 + 0.436 in normal rectal tissue(The Media is 0.00,the maximum is 1.0, the minimum is 0). MLD was higher in rectal cancer than in rectal polyps and normal rectal tissue(P<0.05). MVD was 80.10±23.178in rectal cancer , 27.00± 11.010 in rectal polyps and 10.45±5.337 in normal rectal tissue. MVD was higher in rectal cancer than in rectal polyps and normal rectal tissue(P<0.05). |
