Study On The Molecular Epidemiology Of Methicillin Resistant Staphylococcus Aureus In Nosocomial Infection

Objective To explore the epidemiological feature of Methicillin resistant Staphylococcus aureus (MRSA) by the phenotyping and genotyping method so as to analyze the transmission and mechanism of nosocomial infection caused by MRSA and to reduce its incidence rate. Further appreciate the application value of pulsed-field gel electrophoresis (PFGE) and infre-quent-resistant-site PCR (IRS-PCR) in the genotyping of MRSAMethods 19 MRSA strains isolated and collected from a hospital during september-October in 2004 were analyzed their antimicrobial susceptibility to 16 kinds of drugs, using the similarity coefficient analysis of their antibiosis pattern to perform the phenotyping analysis. PFGE and IRS-PCR of these strains were used to make them genotyping and to reflect the correlation and homology among the isolates using the clustering analysis at the same time.Results The phenotyping results denoted these 19 strains could be divided into four types, 10 strains belonged to type I, 7 strains belonged to type II, the other two strains belonged to type III and type IV respectively. These strains could be divided into 6 types by PFGE and IRS-PCR. Some isolates with high homology was found in 19 MRSAs, 11 isolates of them belonged to subtype A1 (including 8...