The Molecular Epidemiology Of Methicillin-resistant Staphylococcus Aureus In China In 2006

The Objective of thesis is to investigate the antibiotic resistance and molecular epidemiology of methicillin-resistant Staphylococcus aureus in China in 2006. The Methods are that from January to December 2006, 302 consecutive and non-repetitive methicillin-resistant Staphylococcus aureus were collected from 17 teaching hospitals in 15 areas. Antibiotic resistance was studied by agar dilution method. The genotypes of SCCmec were determined by multiplex PCR, Multilocus sequence typing (MLST) was used to type the house-keeping genes. The implementation of the spa typing method was straightforward, and the results obtained were reproducible, unambiguous, and easily interpreted.:The Results of thesis is that The resistant rate of Ciprofloxacin,Levofloxacin, gatifloxacin and erythromycin were higher in MRSA. All of MRSA strains were susceptible to vancomycin . all areas but Dalian harbored SCCmecIII most, while Dalian harbored SCCmecII most. There were two stains in Guangzhou, which was harbored SCCmecâ…£. Spa typing has 14strains, t30accounted for 47.4%; t37accounted for 26.2%; t2accounted for 10.9%; t632 accounted for2.3%; t437 accounted for1.3%; t570,t601accounted for0.7%; t377,t459,t796,t899,t1152,t2649accounted for0.3%. Sequence type(ST) were found in4strains, with ST239accounted for46.7%; ST5 accounted for44.4%; ST59 accounted for6.7%; ST88 accounted for2.2%. The Conclusion is that most types of SCCmec in China is III and II, Spa typing is t30,t37, MLST typing is ST239,ST5, and differed among regions. There existed remarkable difference of MRSA isolate rate and molecular typing. We have found CAMRSA in Guangzhou for 2 strains.