The Expression Of P53 Gene In The Rats Spinal Cord Induced By Combined Treatment With Cisplatin And/or Docetaxel And Radiation

Objective: P53 gene involves in cell cycle regulation. In the normal conditions, the p53 protein exists by the low concentration in the body tissue. When the point mutation, loss and inactivation occurring to p53 gene, through the molecular examination method, may detect the abnormal expression of the p53 protein. At present the association of chemotherapy (CT) and radiotherapy (RT) has been applied widely for the treatment of carcinoma. When the tissues receive the ionization radiation, the chemotherapy medicine and so on should stir up the injury, then p53 is activated and the maintenant the stable expression, which displaying the cell cycle monitoring, promoting the apoptosis of the cells, and guarding against the canceration[1]. Although the combination of CT and RT has obvious improvement on the local control rate, comparing to the single treatment, it increases the toxic effect on the critical normal tissues, inducing various complications. Especially, during the carcinoma therapy involving organs such as head/neck, esophagus, phinophargnx cancer, lung and spinal column, the spinal cord has been irradiated inevitably for a higher dose, even nearing its tolerance. The data of spinal cord injury induced by chemoradiotherapy is quite scarce, because as a late phase injury with manifestation appeared several months or years after the therapy. In this experiment, the spinal cord injury model was established on rats through imitating clinical chemoradiotherapy. Then we observed whether combination of cisplatin/docetaxel and radiotherapy would deteriorate the injury on spinal cord. Determines the expressions of p53 through immunohistochemistry, thus discuss the influence of...