| Background: Microvascular autologous submandibular gland (SMG) transplant is a lasting and effective solution for severe cases of keratoconjunctivitis sicca. Most of Patients with a vital transplant reported a strong relief of symptoms. But after the transplantation operation, some patients were unsuccessful for occlusion of tear drainage duct during the secretory"latent period"; while other patients may have epiphora after the secretory"latent period". Microvascular autograft came through ischemia reperfusion and denervation. It has been proved that apoptosis compact tightly with ischemia reperfusion and denervation in resent references, However, little is known about the apoptosis of microvascular autologous transplanted submandibular gland.Objective: To investigate the apoptosis in vascularized autologous submandibular gland transfer.Methods: Modle of autotransplanted SMG in rabbit was established. SMGs were removed and weighted on post operative day (POD)1, 3, 7, 30, 60, 90. Morphologic features were evaluated by light microscope (LM) and transmission electron microscope (TEM). Expression of caspase 3 (p20) were determined by immunohistochemistry (IHC) and Western blot.Result: The weight of autotransplanted SMG decreased on POD 7 and markedly decreased on POD 30. Under LM, atrophy occurred in the early period after transplantation and the histomorphologic features were recovered on POD 60-90. Obvious apoptotic features were observed in transplanted SMG by TEM. Immunohistochemical stain for caspase3 was increased on POD 7. Caspase3 were mainly expressed in acinar cells of...
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