Experimental Study Of The Effects Of Different Growth Factors On Perfused Rabbits' Kidneys In Vitro At Room Temperature

Chronic renal failure as a common clinical disease severely affects the quality of patients'life. Kidney transplantation has still been an important treatment at present. The demand of renal donor has increased now. However, on one hand, there is a serious shortage of renal donor available, on the other hand, the limitation of the preservation methods results in the wastage of many renal donors. Therefore, it is an urgent task for us to research, find and use an optimal preservation means to minimum the damage of warm ischemia in the course of preservation, prolong the preservation time and increase the utilization rate of renal donor. Our experiment aimed at investigating the effects of different growth factors in different doses on the extracorporeal perfused renal at room temperature.Materials and Methods:36 healthy New Zealand rabbits were randomly divided into 6 groups, according to the different culture media involving growth factors-free(group A) , epidermal growth factor(EGF) in low dosage(group B),EGF in high dosage(group C),hepatocyte growth factor(HGF) in low dosage(group D) , HGF in high dosage(group E), bovine serum(complement was inactivated at 56℃for 30 minutes)(group F). The culture media involving different growth factors were used to perfuse rabbits'kidneys in vitro at room temperature. Each group had 6 rabbits'kidneys. One in each group was used to observe the longest preservation time and the other 5 kidneys in each group were used to measure different biochemical indexes, observe the histological changes under light microscope and the expression of proliferating cell nuclear antigen(PCNA). 6 rabbits'left kidneys in each group were taken out by germfree surgical operation and perfused with autologous blood anticoagulated by heparin and diluted with the equal volume of RPMI 1640 culture medium substrate in vitro at room temperature. Different growth factors in different doses were added to culture media in different groups. On the 24th, 48th, and 65th hours of perfusion, activity of lactate dehydrogenase(LDH),concentration of sodium and potassium in kidney...