A New Method Of Electrophysiological Measurement For The Sphincter Of Oddi In Animals

We have retained the term " sphincter of oddi " used by Ruggero Oddi to designate the duct segment located between the CBD-PD junction and the duodenum for one hundred years.But many diseases from SO remain incompletely understood. Previous studies have been hampered by the fact that the SO segment is too small and it is located largely within the duodenal wall and pancreas in most animal species. Such anatomy makes it difficult to record SO motor activity without interference from duodenal motor activity. In this study our Objective is to explore a new method of electrophysiological measurement for the SO in animals and analyze the myoelectric activity recorded from the SO.The subject consists of two parts as follows:1.New method of electrophysiological measurement for the SO in rabbitspurpose:To explore a new method of electrophysiological measurement for the SO.Material and method:The contact electrode on mucosa were made of two silver wires insulated with insullac and gel silica catheter.The terminal 1 cm of each silver wire was scraped free of insulation. Subsequently, the bared area was surrounded ahead the gel silica catheter. Thirty adult rabbits were divided into two groups with 15 in each group. The rabbits in the first group were inserted the contact electrode on mucosa through duodenum peripapilla and record myoelectric activity of the SO. In the second group the electric activity was recorded by the chorion claw electrode(CCE).In another six rabbits concurrent recordings of myoelectric activity was recorded by two methods.Results:The rate of success of electrophysiological measurement in rabbit could be increased by using CEM. The electric activity recorded by the CEM was nearly the same pattern with the CCE group.