Detection Of Hyperdiploid Malignant Cells In Breas Cancer With A-satellite DNA Probe Of Chromosome 8 And Fluorescence In Situ Hybridization

Breast cancer is woman's common malignant tumor. Approximately 12,000,000 women contract breast cancer and 500,000 ones die of this serious disease every year in the world. The disease incidence rate and the mortality rate by Western Europe, North America high occupy the first place. In our country, in the recent 30 years, breast cancer rose nearly 2 times in the Shanghai female's disease incidence rate, reaches as high as 60/105,Already approached the developed country. The cause of this disease mainly involves heredity,environment,endocrine and virus,etc.It is reported in many articles that aneuploids are observed in the chromosomes of malignant tumor cells. When it comes to the breast cancer, The hyperdiploid state of chromosome 8 is studied outstandingly and most valuable for early diagnosis of breast cancer.Objective To detect the numerical abnormality of chromosome 8 in the breast cancer by fluorescence in situ hybridization(FISH). Methods FISH analysis was performed with the a-satellite DNA probe of chromosome 8 upon 29 clinically collected the breast cancer organize samples, each with clear diagnosis, and the percentage of hyperdiploid cells was calculated, taking normal lymphocytes in peripheral blood as the control. In addition, the result was also compared with relevant clinical diagnosis. results In the normal comparison lymphocytes control, the chromosome 8 a-satellite probe appears two hybrids signals. While hyperdiploid presented in the cells from tumor patients. Hyperdiploid of chromosome 8 was respectively found in 13(68.4%) of 20 malignant breast cancer samples. While for 10 benign breast cancer samples, 9(90%) normal diploid samples were confirmed respectively by each a-satellite DNA probe. Conclusion Hyperdiploid of chromosome 8 is the main characteristic of breast cancer cells from tumor patients. FISH analysis with a-satellite DNA probe of chromosome 8 could detect the hyperdiploid tumor cells in breast cancer samples. To study the interphase cytogenetic difference between benign and malignant breast cancer with FISH assay is convenient, rapid, sensitive and may have novel perspective. And be used as a complentary technique...