Relationship Between NO,NOS And Neurobehavioral Function Changes Induced By Benzo [a] Pyrene

Objective To explore the coincident levels of nitric oxide (NO) ,inducible nitric oxide synthase (iNOS) and neurobehavioral function changes induced by benzo[a]pyrene (BaP) in coke oven workers and rats.Methods 1. Health adult male coke oven workers were selected from a country-owned coke plant , and were classified into three groups according to exposed dose of benzo[a]pyrene (BaP) in the work site , control group were selected from workers in the automation department of the same plant.1)concentration of BaP in the working environment was measured .2)Blood and urine samples were collected immediately after working shift.3) according to the same criteria General information of all subjects correlated with the investigation was collected by query and filled in the questionnaire.4)WHO recommended (neurobehavioral core test battery)NCTB were performed on coke oven workers and controls to test the neurobehavioral changes and the mood state.5)The level of urinary 1-hydroxypyrene(1-OhP) was determined by High Performance Liquid Chromatography (HPLC).6)Levels of NO,iNOS in blood were detected by test kits.2.Forty male SD rats, adult and healthy, were divided into 4 groups randomly: normal oil control, BaP1.3 mg/kg,BaP3.2 mg/kg,BaP7.8 mg/kg. all groups were received intraperitioneal injection for 60d..1)The learning and memory function was measured by Morris water maze.2)The morphology of neurocyte in rats'hippocampus was observed by light microscope.3)The supermicrostructure of neurocyte in rats'hippocampus was observed under transmission electric microscope(TEM).4) Levels of NO,iNOS in rats'blood and hippocampus were detected by test kits.Results 1.1)No significant differences were found among four groups in age, length of service, smoking and drinking. However, there was significant difference in education; There were no significant differences in the scores of the symptomes of digestive system(SDS) and cardiovascular system(CVS) (P>0.05).However, there were significant differences in the scores of the symptomes of nervous system(SNS), the symptomes of endocrine system(SES)...