Comparison Of Protective Effects Of Cariporide And Verapamil On Myocardial Ischemia Reperfusion Injury In Rats

Objective: To compare the protective effects of Cariporide and Verapamil on myocardial ischemia reperfusion injury.Methods: Thirty Wistar rats(weights 250～290g) were randomly divided into five groups(n=6 each group): GroupⅠ, normal group; GroupⅡ, ischemia-reperfusion injury group, subjected to 30min occlusion and 60min reperfusion; GroupⅢ, apply Cariporide group; GroupⅣ, apply Verapamil group; GroupⅤ, apply Cariporide&Verapamil group. Rats ischemia reperfusion model was established by ligation of left anterior descending coronary. GroupⅢ,Ⅳ,Ⅴ, also subjected to 30min occlusion and 60min reperfusion, Cariporide and Verapamil and Cariporide&Verapamil were given iv after 30min myocardium ischemia respectively. The changes of voltage of ST of ECG inⅡ-lead and amount of Cardiac Troponin I(cTnI) and Myoglobin(Mb) in plasma on acute ischemia and reperfusion were examined. Myocardial ultrastructures were examined under transmission electron microscope.Results: The amount of Mb in plasma had no significant difference among each groups(P>0.05). The amount of cTnI in plasma in groupⅢ,Ⅳ,Ⅴwere lower than that of ischemia-reperfusion injury group(P<0.05), but had no significant difference among the groupⅢ,Ⅳ,Ⅴ(P>0.05). The changes of voltage of ST inⅡ-lead electrocardiogram were significant difference between groupⅡand groupⅢ(P<0.05), but had no significant difference between groupⅡand groupⅣ(P>0.05), and between groupⅢand groupⅣ(P>0.05). The myocardial ultrastructural changes were significant in groupⅡ, there were significant edema in myocardium and in mitochondria, severe damage in mitochondria; but there were not so severe in groupⅢ,Ⅳ,Ⅴ.Conclusion: Both Cariporide and Verapamil show protective effects on myocardial ischemia reperfusion but the protective effect of Cariporide was no better than that of Verapamil. Cariporide&Verapamil combined apply had no better protective effect than that of single applying one of the two.