Effects Of Genistein And Diethylstilbestrol On Bone Metabolism In Orchidectomized Rats

Objective To investigate the effects of genistein and diethylstilbestrol on bone metabolism in orchidectomized rats.Methods 40 male Sprague-dawley rats,3 to 4 months old,were randomly allocated into five groups: sham-operate(Sham), orchidectomized(Orch), orchidectomized supplied diethylstilbestrol (DES, 5μg/d) or genistein (GenⅠ1mg/d,GenⅡ2mg/d).After 16 weeks with low calcium feed , the serum concentration of osteocalcin(BGP), testosterone(T), Estradiol(E2), alkaline phosphatase(AKP) and the urinary contents of pyridine(PYR) and hydroxyproline(HYPRO) were analysed.Results Orchidectomy reduced serum estradiol and testosterone concentrations. The decrease of testosterone was significien(tP<0.05).The serum concentrations of BGP,urinary contents of PYR and HYPRO of the Orch rats were increased significiently(P<0.05)and serum concentrations of AKP were increased slightly campared with the Sham rats. After 16 weeks with genistein or diethylstilbestrol supplementation, the serum BGP,urinary PYR and HYPRO were improved significiently in orchidectomized rat(sP<0.05). Serum estradiol and testosterone were not affected significiently.Conclusions Genistein(1mg/d or 2mg/d) and diethylstilbestrol(5μg/d) improved biochemical parameters of bone metabolism.The findings of this study suggest that genistein may have positive effects on the male skeleton.