Effect Of Smoking On Reproduction In Male Mice

Objective: To study the effect of smoking on male reproduction. Methods: 110 Kunming male mice were selected, and 10 were killed randomly after a week of adjustable feeding and varied indexes were observed. The 100 male mice left were randomly divided into smoking group and compared group, 50 in each group. Smoke was in haled by the mice in the smoking group for 1 hour per day and the compared group inhaled natural air. The smoking group and the compared were respectively divided into 5 sub-groups, i.e. sub-group of 2 weeks inhalation, sub-group of 4 weeks inhalation, sub-group of 6 weeks inhalation, sub-group of 8 weeks inhalation and the reproduction group with 10 male mice in each group. By the end of 2^ed, 4^th, 6^th, 8^th week 10 male mice in the smoking group and the compared group were respectively killed and the index were observed. After being exposed to smoke for 8 weeks, the reproduction group male mice mated with female mice in 1 : 1 for 1 week, and the exposure continued during mating. Pregnant female mice naturally labored, and the experiment ended after the observation of their offsprings for 21 days. Results: 1) With the extension of exposed time of smoke, the increase of weight in the smoke group and the organ coefficients of testes, epididymis, and seminal vesicles were apparently lower than those of the compared group, especially in the group of 8 weeks inhalation (P<0.05). The rate of abnormal sperm increased with the extension of the exposed time, especially in the group of 8 weeks inhalation (P<0.05). 2) The micro structure of testes contracted to some degree in the smoke group. 3) The activity of SOD,MDA and NOS of testis gradually increased with the extension of exposed time (.P<0.05), especially in the group of 6 and8 weeks inhalation in which the differences were significant between the smoking group and the compared group(P<0.05 or P<0.01). While the activity of ACP and LDH of testis...