Study On The HPLC Fingerprint And The Active Components In Echinacea

Echinacea is a traditional medicinal herb in North American perennial. It can enhance the body immune system and antagonize various kinds of infections. In recent years, Echinacea can be widely used in Germany, Canada, America and so on, and has becomes one of ten internationally popular herbs.High-performance liquid chromatography (HPLC) coupled with photodiode array detection (DAD) techniques was used to set up the fingerprint chromatogram condition for the analysis of different kinds of Echinacea species. The analyses were carried out on a Shiseido RP-C18 column (250mm×4.6mm i.d,5μm). The mobile phase was (A) aqueous trifluoroacetic acid solution (0.2%) solution and (B) acetonitrile trifluoroacetic acid solution (0.1%). The gradient elution was modified as follows: initial 10% B; 0-35min, 10-25% B; 35-45min, 25-50% B; 45-50min, 50-70% B and hold for 3min; 53-55min, 70-10% B. The flow rate is 1.0mL/min. The column oven temperature was set at 30 C. Detection wavelength was set at 329nm. The sample injection volume was 20μL.HPLC fingerprint techniques was used for integrate evaluation of multiple active components in different parts of Echinacea different species from different cultivation areas. The flowers are selected to construct the model for the standard fingerprint of Echinacea purpurea. The Max-plot function of the DAD and Evaporation light scattering detector (ELSD) were utilized to evaluate the...