Expression Of BP1 In Acute Leukemia And Its Significance

[Objective] To investigate the expression and its significance of BP1 mRNA in acute leukemia patients.[Methods] BP1 level was assessed by reverse transcription polymerse chain reaction (RT-PCR) in 64 adult acute leukemia patients with newly diagnosed, 4 patients relapsed, 7 healthy volunteers and 5 patients with ITP (Idiopathic Thrombocytopenic Purpura), as well as leukemia cell line K562. The regularity of the expression of BP1 gene in acute leukemia and the relationship between BP1 level and the prognosis of leukemia was explored.[Results] BP1 mRNA was expressed in 47.9 % (n=23 ) AML patients and 6.25% (n=l) ALL patients with newly diagnosed. The expression of BP1 mRNA was positive in leukemia cell line K562, but negative in 7 healthy donors and 5 ITP patients. BP1 expression was neither correlated to the age, sex and FAB type of patients, nor to the white blood count, hemoglobin, platelet count and the percentage of blasts at diagnosis. The level of BP1 mRNA was related to curative effect of AML patients. The complete remission group appeared having a lower BP1 mRNA expression than non-remission and die group (P<0.05 ) . BP1 mRNA was reappeared in patients prior to their relapse who had received regularly chemotherapy.[Conclusions] BP1 mRNA was overexpressed in adult acute leukemia patients and leukemia cell lines. The level of BP1 mRNA was related to curative effect of AML patients. The expression of BP1 may be a mark of adult AML and an indicator of prognosis. The significant differential expression between adult acute leukemia...