| Objective: To investigate the effect of chronic mild unpredictable stress on rats in general activity, emotional state, reward responsiveness, spatial memory and neuroimmunoendocrine.Methid: 24 adult Male Sprague-Dawley rats were selected and devided into two groups:stress and control group randomly, according to their body weight. Stressed rats were exposed to 8 weeks of different stressors applied at various times. During the whole procedure, stressed and non-stressed rats were tested in open field box, in MWM,and were tested for sucrose preference.Serum ACTH, serum CORT, TNF, IL-1β, IL-2 were determined using a radioimmunoassay.Result: 1, Body weight and daily food intake of stressed rats were decreased significantly from 1st week to 8th week (P<0.01) ;2> In open-field test, squares crossed of stressed rats were reduced significantly from 4th to 7th week, rears were reduced significantly from 6th to 7th week( P<0.05),grooming time were increased significantly in the 7th week (P<0.05) ,there was no difference between two groups on central time and defecation.3, In sucrose consumption test, sucrose cunsumption, preference for sucrose and sucrose consumption per gram body weight revealed no significant difference between two groups; 4, In MWM latency test, there was no difference between two groups on the time to reach the hidden or visible platform. In probe test, the duration of the time spent in the correct quadrant did not differ.5, There was no difference between two groups on serum ACTH, TNF, IL-1β, IL-2. Serum CORT of rats exposed to stress was reduced significantly (p<0.05) .There was negative correlation between serum CORT and IL-2 or between IL-1 β and IL-2 in control group, but not in stressed group.Conclusion: After being exposed to chronic mild unpredictable stress, rats...
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