Effects Of GM-CSF On Acute Lung Injury Following Hemorrhage Shock In Mice

OBJECTIVE To investigate effects of the specific GM-CSF-neutralizing antibody (22E9) and dexamethasone on acute lung injury following hemorrhage shock in mice. Dexamethasone and different dose 22E9 was instilled mouse nose respectively in the period of hyperventilation before hemorrhage shock. Pathology, NF-κB activation,TNF-α, the ratio of wet/dry (W/D) and TLR4 expression in pulmonary macrophages were detected in lungs of different groups after hemorrhage-induced acute lung injury. In addition, NF-κB activation in hearts, livers, kidneys and TNF-α in hearts were detected at 4h after hemorrhage-induced acute lung injury in different groups.METERIALS AND METHODS Seventy-five male C57BL/6 mice were used to reproduce model of sham and hemorrhagic shock with different nterventions.Which were randomly divided to five groups including negative control group (NCG), hemorrhagic shock alone group (PCG), hemorrhagic shock group with intervention by lug/mouse 22E9 (HS1), hemorrhagic shock group with intervention by 10ug/mouse 22E9 (HS10), hemorrhagic shock group with intervention by 20ug/mouse dexamethasone (DEX). Solution (35ul) was applied to the nares in the period of hyperventilation before cardiac puncture, which administered respectively to the mice of different groups alone or in combination were in turn as follows: 1) PBS;2) PBS; 3) PBS containing lug 22E9; 4) PBS containing 10ug 22E9; 5) PBS containing 20ug dexamethasone .The animals were killed and lungs,hearts,livers, kidneys were harvested at 0, 1, 2, 4 and 6 h after hemorrhagic shock. The ratio of W/D was measured directly. The NF-kB activation of lungs,hearts,livers,kidneys...