The Investigation Of "Myocardium-like" Cell Conversion From Bone Marrow Adult Stem Cells

Aim: Acute myocardial infarction was one of the main diseases harming human healthy. Although maturity cardiomyocytes were not terminally differentiated cells, they had limited split and prliferation ability. Scar tissue took the place of the destroyed myocardium after myocardiac infarction because of the very low regenerative potentiality of cardiomyocytes. Ventricle remodeling and heart failure occured accordingly, died ultimately. Nowadays, acute myocardial infarction therapeutic methods include thrombolysis, percutaneous transluminal coronary angioplasty (PTCA), coronary artery bypass graft (CABG), and heart transplantation. Cell transplantation therapy was a new method to treat myocardiac infarction. The adult stem cells had high potentiality of duplication and differentiation, they play an active role in improving cardiac function after implantation by angiogenesis and differentiating into cardiomyocytes. The adult stem cells used in heart diseases include bone marrow stem cell, skeletal muscle satellite cell, smooth muscle stem cell, embryonic stem cell and fetus cardiac myocyte. Bone marrow stem cells distributed widely, animmunologic rejection in implantation, no ethics altercation, therefore they have superiority in cellular myocardium mending. This investigation would discuss bone marrow stem cells were converted into myocardium-like cells induced by 5-azacytidine or Panax notoginosides in vitro, and discuss the effection of "myocardium-like" microenviroment to this process.Method:1. Marrow mesenchymal stem cells (MSCs) were induced into "myocardium-like" cardiomyocytes in vitro.(1) MSCs from adult rats were isolated and cultured in cell culture medium.(2) The MSCs were treated by 5-azacytidine (1, 5, 10, 20μmol/L) for 24 hours. At...